Polyoma virus middle

Shimotohno, K. Laskey, R. Shinnick, T. Rowe, W. Virology 42 , — Papkoff, J. Virology , 91— Deininger, P. Nucleic Acids Res. Donoghue, D. Acids Res. Maxam, A. Bina-Stein, M. McClure, D. Download references. You can also search for this author in PubMed Google Scholar. Reprints and Permissions. Transmission of the polyoma virus middle T gene as the oncogene of a murine retrovirus. Download citation. Received : 20 September Accepted : 22 February Issue Date : 19 April Characterization of polyoma virus T antigen.

Middle T antigen as primary inducer of full expression of the phenotype of transformation by polyoma virus. Construction and analysis of viable deletion mutants of polyoma virus. Simian virus 40 functions required for the establishment and maintenance of malignant transformation. Roles of the simian virus 40 tumor antigens in transformation of Chinese hamster lung cells: studies with simian virus 40 double mutants.

The roles of the simian virus 40 tumor antigens in transformation of Chinese hamster lung cells. Simian virus 40 gene A function and maintenance of transformation. An enzymatic function associated with transformation of fibroblasts by oncogenic viruses. J Exp Med. Distinct transformation phenotypes induced by polyoma virus and simian virus 40 in rat fibroblasts and their control by an early viral gene function.

Plasminogen activator production accompanies loss of anchorage regulation in transformation of primary rat embryo cells by simian virus Cell-free translation of simian virus 40 early messenger RNA coding for viral T-antigen. Growth control in simian virus transformed rat cells: temperature-independent expression of the transformed phenotype in tsA transformants derived by agar selection.

Conditions leading to the establishment of the N a gene dependent and A a gene independent transformed states after polyoma virus infection of rat fibroblasts. The genome of simian virus The transforming genes of SV40 and polyoma.

Sensitive proteolytic enzyme assay using differential solubilities of radioactive substrates and products in biphasic systems. Anal Biochem. Tumor antigen s in cell productively infected by wild-type polyoma virus and mutant NG The association of PtdIns 3-kinase with the mT-pp60c-src complex was dependent on the protein-tyrosine kinase activity of pp60c-src as a kinase-inactive mutant pp60 c-src still complexed with mT, but the mT-pp60 c-src complex was unable to bind PtdIns 3-kinase.

The mT-pp60c-src complex phosphorylated both subunits of PtdIns 3-kinase on tyrosine residues. The immunopurified mT-pp60c-src complex also associated with PtdIns 3-kinase activity from whole cell lysates, and this association was dependent upon the protein-tyrosine kinase activity of pp60c-src. Eur J Biochem. Cytoskeletal elements of chick embryo fibroblasts revealed by detergent extraction.

Association of the src gene product of Rous sarcoma virus with cytoskeletal structures of chicken embryo fibroblasts. Carboxy terminus of polyoma middle-sized tumor antigen is required for attachment to membranes, associated protein kinase activities, and cell transformation. Membrane isolation on polylysine-coated beads. Plasma membrane from HeLa cells. J Cell Biol. A microspectrophotometric method for the determination of cytochrome oxidase. Pyruvate carboxylase: affinity labelling of the magnesium adenosine triphosphate binding site.

An activity phosphorylating tyrosine in polyoma T antigen immunoprecipitates. In vitro synthesis of a functional avian sarcoma virus transforming-gene product. Evidence that the avian sarcoma virus transforming gene product is a cyclic AMP-independent protein kinase. Characterization of protein kinase activity associated with the transforming gene product of Fujinami sarcoma virus.

Localization of gene functions in polyoma virus DNA. Rous sarcoma virus transforming protein, p60src, expressed in E. Binding of insulin to isolated nuclei.

The location of proteins labeled by the I-lactoperoxidase system in the NIL 8 hamster fibroblast. New classes of viable deletion mutants in the early region of polyoma virus. Interactions of a photo-affinity ATP analog with cation-stimulated adenosine triphosphatases of human red cell membranes. Localization of the high-affinity ATP site in adenosine-3':5'-monophosphate-dependent protein kinase type I.

Photoaffinity labelling studies with 8-azidoadenosine 5'-triphosphate. Transforming gene product of Rous sarcoma virus phosphorylates tyrosine. Characterization of T antigens in polyoma-infected and transformed cells. Polyoma virus-specific 55K protein isolated from plasma membrane of productively infected cells is virus-coded and important for cell transformation.

Virus-specific proteins in the plasma membrane of cells lytically infected or transformed by pol-oma virus.